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目的探讨CYP1A1遗传多态性与广西壮族原发性肝癌遗传易感性关系。方法以60例肝细胞癌(HCC)患者(30例汉族、30例壮族)及15例非肝癌患者为研究对象,取肝癌组织和非肝癌患者肝组织标本提取DNA,用PCR-RFLP法检测CYP1A1基因多态性及基因突变分布频率。结果 (1)肝癌组和对照组CYP1A1基因突变率分别为38.3%(23/60)、6.67%(1/15)(P=0.041,OR为3.824)。(2)汉族肝癌和壮族肝癌患者CYP1A1基因突变率分别为36.7%(11/30)、40.0%(12/30)(P=0.791)。结论 (1)CYP1A1基因突变更易发生肝癌,其危险性是CYP1A1基因未突变者发生肝癌的3.824倍。(2)CYP1A1基因型在汉族、壮族肝癌患者中分布无明显差异。
Objective To investigate the relationship between CYP1A1 genetic polymorphism and genetic susceptibility to primary hepatocellular carcinoma in Guangxi Zhuang Autonomous Region. Methods Sixty patients with hepatocellular carcinoma (HCC) (30 Han, 30 Zhuang) and 15 non-HCC patients were enrolled. DNA was extracted from liver tissues of patients with hepatocellular carcinoma and non-HCC and PCR-RFLP was used to detect the expression of CYP1A1 Gene polymorphism and frequency of gene mutation distribution. Results (1) The mutation rates of CYP1A1 in liver cancer group and control group were 38.3% (23/60) and 6.67% (1/15) respectively (P = 0.041, OR = 3.824). (2) The mutation rates of CYP1A1 gene in Han and Zhuang patients were 36.7% (11/30) and 40.0% (12/30), respectively (P = 0.791). Conclusions (1) CYP1A1 mutation is more likely to cause liver cancer, the risk of CYP1A1 gene is not mutated 3.824 times that of liver cancer. (2) CYP1A1 genotype distribution in Han and Zhuang patients with no significant difference.