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目的 为了探讨P53蛋白和增殖细胞核抗原在食管、贲门癌中的表达及其临床意义。 方法 应用SP免疫组织化学法,研究了40例食管鳞状细胞癌和28例贲门腺癌P53蛋白的表达及其与细胞增殖活性、淋巴结转移的关系。 结果 食管癌与贲门癌P53蛋白阳性率分别是60%和57.1%。53.3%的癌旁组织中有P53蛋白过度表达。食管癌有淋巴结转移者P53蛋白阳性率和细胞增殖活性较无转移者明显增高(P<0.01),说明p53基因的突变以及导致细胞恶性增殖不仅与食管、贲门癌的发生有关,而且在其淋巴结转移中也起重要作用。 结论 检测P53蛋白和细胞增殖核抗原对食管、贲门癌的早期诊断及判断肿瘤的恶性程度,评估预后有较高的参考价值
Objective To investigate the expression of P53 protein and proliferating cell nuclear antigen in esophagus and cardia cancer and its clinical significance. Methods SP immunohistochemical method was used to study the expression of P53 protein in 40 esophageal squamous cell carcinoma and 28 gastric cardia adenocarcinoma and its relationship with cell proliferation activity and lymph node metastasis. Results The positive rates of P53 protein in esophageal and gastric cardia were 60% and 57.1% respectively. P53 protein was overexpressed in 53.3% of paracancerous tissues. P53 protein positive rate and cell proliferation activity in patients with esophageal cancer with lymph node metastasis were significantly higher than those without metastasis (P <0.01), indicating that p53 gene mutation and lead to malignant proliferation of cells not only with the occurrence of esophageal and cardiac cancer, but also in the lymph nodes Transfer also plays an important role. Conclusion The detection of P53 protein and cell proliferation of nuclear antigen for early diagnosis of esophageal and gastric cardia cancer and to determine the degree of malignancy of the tumor to assess the prognosis of a higher reference value