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睡莲(Nymphaea spp.)为多年生水生观赏花卉。以耐寒睡莲不同花色的119个栽培品种为材料,利用高效液相色谱(HPLC-DAD)和液质联用技术(HPLC-ESI-MSn)测定了其花瓣中的花青素苷成分。采用英国皇家园艺学会比色卡(RHSCC)和国际照明委员会(CIE)制定的CIEL*a*b*表色系统测量了57个品种的花色,运用多元线性回归方法分析花色与花青素苷组成之间的关系。结果表明:耐寒睡莲花瓣中含有14种花青素苷,其中飞燕草素-3-半乳糖-5-乙酰-半乳糖苷(Dp3Ga5acetyl Ga)、飞燕草素-3-鼠李糖-(1→2)-半乳糖苷(Dp3Rh(1→2)Ga)、矢车菊素-3-半乳糖-(1→2)-半乳糖苷(Cy3Ga(1→2)Ga)、矢车菊素-3-乙酰-半乳糖-(1→2)-半乳糖苷(Cy3acetylGa(1→2)Ga)、矢车菊素-3-没食子酰-半乳糖苷(Cy3galloylGa)、飞燕草素-3-乙酰-葡萄糖苷(Dp3acetylG)、飞燕草素-3-葡萄糖苷(Dp3G)和矢车菊素-3-半乳糖-半乳糖-半乳糖苷(Cy3GaGaGa)8个组分在耐寒睡莲中为首次报道。Dp3Ga、Dp3galloylGa、Cy3Ga(1→2)Ga和Cy3galloylGa是决定耐寒睡莲呈色的关键花青素苷。
Nymphaea spp. Is a perennial aquatic ornamental flower. A total of 119 cultivars with different color of Lonicera mairei were used as materials. The anthocyanin content in petals was determined by HPLC-DAD and HPLC-ESI-MSn. Fifty-seven varieties of flowers were measured using the CIEL * a * b * color system developed by RHSCC and CIE, and the color and anthocyanin composition were analyzed by multivariate linear regression analysis The relationship between. The results showed that there are 14 kinds of anthocyanin glycosides in the cold-tolerant water lily petals, among which, Dp3Ga5acetyl Ga and delphinidin-3-rhamnose - (1 → 2) -galactoside (Dp3Rh (1 → 2) Ga), cyanidin-3-galactose- (1 → 2) -galactoside (1 → 2) -galactoside (Cy3acetylGa (1 → 2) Ga), cyanidin-3-galloylgalactosamine-3 8 components of Dp3acetylG, Dp3G and Cy3GaGaGa were the first in cold water lily Times reported. Dp3Ga, Dp3galloylGa, Cy3Ga (1 → 2) Ga and Cy3galloylGa are the key anthocyanins that determine the color of Lonicera.