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目的建立测定水和土壤中二氯喹啉酸残留量的高效液相色谱检测方法,为二氯喹啉酸残留研究提供重要技术支持。方法水和土壤经不同溶剂提取浓缩后,经阴离子交换柱净化浓缩,用高效液相色谱-紫外检测器进行定量分析。结果结果表明,二氯喹啉酸经过净化浓缩后,无明显杂质干扰测定,水和土壤的添加浓度在5.0～100μg/kg时,回收率分别在91%～96%、89%～98%,变异系数均小于7%,方法最低检出浓度水中为0.6μg/L、土壤中为1.0μg/kg。结论该方法对水和土壤中二氯喹啉酸残留的检测灵敏度高,重现性好,满足农药残留痕量检测需求。
Objective To establish a HPLC method for the determination of quinclorac residue in water and soil, and provide important technical support for quinclorac residue research. Methods Water and soil were extracted with different solvents and concentrated. The samples were purified by anion exchange chromatography and quantified by high performance liquid chromatography-ultraviolet detector. The results showed that after quinclorac was purified and concentrated, there was no significant interference of impurities. The recoveries were 91% -96% and 89% -98% at water and soil concentrations of 5.0-100 μg / kg, respectively The coefficients were all less than 7%. The minimum detectable concentration was 0.6 μg / L in water and 1.0 μg / kg in soil. Conclusion The method has high sensitivity and reproducibility for the detection of quinclorac residues in water and soil, and meets the requirements of pesticide residue trace detection.