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Expression of transient receptor potential (TRP) channels is widespread with transcripts distributed throughout the brain.All TRP channel subunits are activated following phospholipase C activation and form cation-selective ion channels.Previous studies examining the existence of TRP channels in hippocampal CA1 pyramidal neurons were based on cultured neurons.Therefore,their relevance for living tissue remains unclear.In the present study,patch-clamp recordings were conducted from CA1 pyramidal neurons in hippocampal slices from 7-day-old rats.Whole-cell currents were obtained from CA1 hippocampal neurons with potentiation effects of 2-aminoethoxydiphenyl borate and lanthanum,revealing that recorded experimental currents were characteristic TRP-like channel currents.Identification of rat hippocampal mRNA transcripts of TRPC4,TRPC5,TRPV1,TRPV2,and TRPV3 channels further verified the expression of characteristic TRP-like channels on rat CA1 hippocampal neurons.
Expression of transient receptor potential (TRP) channels is widespread with transcripts distributed throughout the brain. All TRP channel subunits are activated following phospholipase C activation and cation-selective ion channels. Previous studies examining the existence of TRP channels in hippocampal CA1 pyramidal neurons were based on cultured neurons. Before, their relevance for living tissue remains unclear. the present study, patch-clamp recordings were conducted from CA1 pyramidal neurons in hippocampal slices from 7-day-old rats. Well-cell currents were obtained from CA1 hippocampal neurons with potentiation effects of 2-aminoethoxydiphenyl borate and lanthanum, revealing that the recorded experimental currents were characteristic TRP-like channel currents. Identification of rat hippocampal mRNA transcripts of TRPC4, TRPC5, TRPV1, TRPV2, and TRPV3 channels further verified the expression of characteristic TRP -like channels on rat CA1 hippocampal neurons.