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瓜果腐霉菌Pythium aphanidermatum的高等植物寄主已超过59属74种.分离或检出土壤中的瓜果腐霉菌已有几种方法,但它们都不够完善.本法是直接在2%琼脂平板培养基上用黄瓜籽作为诱发基质,加磷酸(PS)盐溶液诱导产生孢子.在直径9厘米的培养皿内,放入10粒黄瓜种子和10克新采的土壤,加入6毫升水,置30—35℃温箱内培养12—24小时,取出种子,在流水下冲洗30分钟后在30分钟内风干.在每皿12毫升2%琼脂培养基平板上放2粒经过上述处理的种子,随后在无菌条件下注入4毫升磷酸盐溶液(配方:KH_2PO_4150毫克,
The higher plant host of Pythium aphanidermatum has more than 59 genera and 74 species. There are several ways to isolate or detect Pythium melon in the soil, but they are not perfect enough. This method is directly on 2% agar plate culture Cucumber seeds were used as the substrate for the induction, and spores were induced by the addition of phosphoric acid (PS) salt solution. In a 9 cm diameter petri dish, 10 cucumber seeds and 10 g newly collected soil were added, 6 ml of water was added, After incubation for 12-24 hours at -35 ° C in the incubator, seeds were removed and rinsed for 30 minutes under running water and air-dried within 30 minutes. Two seeds treated as above were placed in 12 ml 2% agar plates per plate Inject sterile 4 ml phosphate solution (formula: KH_2PO_4150 mg,