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目的优选柴芎感冒合剂的水提醇沉工艺条件,为该制剂的开发提供依据。方法以挥发油得率为指标,采用单因素试验,考察浸泡时间、加水倍量、煎煮时间对挥发油提取工艺的影响;以柴胡皂苷的总量及干膏率的综合评分为指标,采用正交试验考察提取时间、提取次数及加水倍量对柴芎感冒合剂提取工艺的影响;采用单因素试验,以除杂率、柴胡皂苷的保留率为指标,考察乙醇浓度及相对密度对醇沉工艺的影响。结果挥发油提取工艺为加10倍水,水蒸气蒸馏提取6 h;最佳水提工艺为加8倍量水提取2次,每次1 h;优选的醇沉工艺条件为提取液浓缩至相对密度1.15,加入乙醇使含醇量为65%,静置24 h除去沉淀。结论该工艺稳定可行,可为其工业化生产提供实验依据。
Objective To optimize the water extraction and alcohol precipitation process conditions of Cynanchum chuanxiong cold mixture, to provide the basis for the development of the preparation. Methods Taking volatile oil yield as an index, single factor experiments were conducted to investigate the effects of soaking time, water addition times and decocting time on the extraction of volatile oil. Using the total score of saikosaponin and dry cream as the index, The effects of ethanol extraction time, extraction times and water addition times on the extraction process of Cynanchum chuanxiong cold mixture were investigated. The single factor test was used to study the effects of ethanol concentration and relative density on ethanol precipitation The impact of technology. Results The extraction process of volatile oil was 10 times of water and extracted by steam distillation for 6 h. The best water extraction process was extraction twice with 8 times of water for 1 h each time. The optimal alcohol precipitation conditions were the concentration of extract to relative density 1.15, ethanol was added so that the alcohol content of 65%, allowed to stand for 24 h to remove the precipitate. Conclusion The process is stable and feasible, which can provide experimental basis for its industrialized production.