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目的:研究西妥昔单抗(Cetuximab,C225)对耐多西他赛(Docetaxel)肺腺癌细胞株SPC-A-1/docetaxel放化疗敏感性的调变作用。方法:克隆形成实验观察C225对SPC-A-1/docetaxel细胞株放疗敏感性的影响;MTT比色法观察C225单独应用以及不同次序联合多西他赛对SPC-A-1/docetaxel细胞株的生长抑制作用;流式细胞术检测C225对SPC-A-1/docetaxel细胞凋亡及细胞生长周期的影响。结果:C225联合放疗可以显著减少SPC-A-1/docetaxel细胞的克隆形成数目,其D_0值以及单纯放疗的D_0值分别为1.73 Gy和2.39 Gy,增敏比为1.38。C225单药即使在高达1 000μg/ml的质量浓度下作用48 h对SPC-A-1/docetaxel细胞无细胞毒和生长抑制作用;在使用多西他赛之后使用C225,多西他赛的IC_(50)值为85.2μg/ml,较单独使用多西他赛时的IC_(50)值128.7μg/ml显著降低。C225单独应用可以诱导SPC-A-1/docetaxel细胞凋亡,并具有时间效应。C225处理(24h)前后G_1期细胞比例分别为(43.80±4.46)%及(60.50±6.57)%(P<0.05)。结论:C225增加了SPC-A-1/do- cetaxel细胞株对放化疗的敏感性,其机制可能与其诱导凋亡及G_1期细胞周期阻滞有关。
Objective: To investigate the effect of Cetuximab (C225) on chemosensitivity to docetaxel-resistant lung cancer cell line SPC-A-1 / docetaxel. Methods: The effect of C225 on radiosensitivity of SPC-A-1 / docetaxel cells was observed by clonogenic assay. MTT assay was used to observe the effect of C225 alone and in combination with docetaxel The effect of C225 on the apoptosis and cell cycle of SPC-A-1 / docetaxel cells was examined by flow cytometry. Results: C225 combined with radiotherapy could significantly reduce the number of colonies in SPC-A-1 / docetaxel cells. The D0 value and radiotherapy D0 value were 1.73 Gy and 2.39 Gy, respectively, and the sensitization ratio was 1.38. C225 single-agent did not have cytotoxic and growth-inhibitory effect on SPC-A-1 / docetaxel cells even after 48 h treatment at mass concentration up to 1 000 μg / ml; C225 after docetaxel administration, IC_ (50) value of 85.2μg / ml, compared with the IC 50 value of docetaxel alone 128.7μg / ml significantly reduced. C225 alone could induce apoptosis of SPC-A-1 / docetaxel cells and had a time effect. The percentage of cells in G_1 phase before and after C225 treatment were (43.80 ± 4.46)% and (60.50 ± 6.57)%, respectively (P <0.05). Conclusion: C225 increases the chemosensitivity of SPC-A-1 / do-cetaxel cells to radiotherapy and chemotherapy, which may be related to its induction of apoptosis and cell cycle arrest in G_1 phase.