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目的研究贯叶连翘提取物体内外对甲1型流感病毒(IAV)活性的影响。方法以细胞存活率和抑制率为指标,采用MTT比色法检测贯叶连翘提取物体外抑制IAV活性的效应;体内实验以流感病毒滴鼻感染小鼠,分别ig给药,观察小鼠死亡率、生命延长率及小鼠病毒性肺炎的改变,判定其抗流感病毒作用。结果贯叶连翘提取物在体外随药物质量浓度的增加其细胞存活率和病毒抑制率明显升高,与病毒对照组相比均有显著性差异(P<0.01);并可降低流感病毒感染小鼠的死亡率,延长存活时间,明显减轻小鼠病毒性肺炎的症状(P<0.01)。结论贯叶连翘提取物在体内外均有良好的抗IAV活性的作用。
Objective To study the effect of extract of Hypericum perforatum on the activity of influenza virus type I (IAV) in vitro and in vivo. Methods The cell viability and inhibition rate were used as indicators to detect the effect of extract of Hypericum perforatum on IAV activity in vitro. MTT assay was used to infect mice in vivo by intranasal injection of influenza virus, Life expectancy and viral pneumonia in mice to determine the role of anti-influenza virus. Results Compared with the virus control group, the cell viability and the virus inhibition rate of Hypericum perforatum extract increased significantly with the increase of the drug concentration in vitro (P <0.01), and could reduce the influenza virus infection in mice Of the mortality, prolong survival time, significantly reduce the symptoms of viral pneumonia in mice (P <0.01). Conclusion Hypericum perforatum extract has a good anti-IAV activity in vivo and in vitro.