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目前,在许多工业和研究部门,高效液相色谱仪是最常用的分析工具之一,因为它的应用范围很广,通过选择不同类型的色谱柱和相应的流动相,以及使用不同的检测器以适合各方面的需要。正因为高效液相色谱仪有很多部件和参数可供选择和调节,故而仅仅简单地操作仪器是不够的,应当准确地掌握和运用这门技术,遇有故障能够正确地判断并及时排除,以下将介绍实验工作中容易出现的问题和解决的办法。
Currently, HPLC is one of the most commonly used analytical tools in many industrial and research sectors because of its wide range of applications, by selecting different types of columns and corresponding mobile phases, and using different detectors To suit all needs. Because of the many components and parameters available for selection and adjustment of the HPLC, it is not enough to simply operate the instrument. Therefore, the technology should be accurately grasped and used. In the event that a malfunction can be correctly determined and eliminated in time, Will introduce the experimental work prone to problems and solutions.