从猪囊尾蚴内提取 m RNA经反转录合成 c DNA,应用定向克隆的方法 ,将合成的 c DNA片段重组到噬菌体载体 Uni- ZAP XR的 Eco R 和 Xho 双酶切位点之间。5 μg poly(A) + RNA所构建的表达文库容量为 0 .98×10 6重组子。经含有 IPTG和 X- gal的颜色选择平皿测定 ,提示重组率达 86 %。随机取 6个噬菌斑做 PCR,检查插入片断的长度在 10 0～ 2 0 2 0 bp之间 The m RNA was extracted from Cysticercus cellulosae and reverse transcribed into c DNA. The recombinant c DNA fragment was recombined into the Eco R and Xho double restriction sites of the phage vector Uni-ZAP XR using directional cloning method. The expression library constructed by 5 μg poly (A) + RNA had a capacity of 0.98 × 10 6 recombinants. The color selection plate containing IPTG and X-gal assay indicated a recombination rate of 86%. Randomly take six plaques PCR, check insert length of 10 0 ~ 2020 bp