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组织学上,把釉质早期龋(白斑损害)分为几层:①透明层,②暗层,③病变本体和④表层。由于以前制备釉质的超薄切片方法往往产生人工损伤,所以透射电镜检查至今还未能提供这四层超微结构变化的资料。本文介绍了一种改进的用氩离子光将选择好的区域制薄技术。这种技术对正常釉质只产生最小的人工损伤。这方法能直接看见釉质的镜下表现,釉质晶体是板条状的,但不能确定其长度。作者从15至30岁青年人选择患有早期近似龋(白斑损害)的24颗恒牙,常规制成磨片,再用氩离子光将选好病变区制薄,进行电镜观查。
Histologically, the early enamel caries (white lesions) is divided into several layers: ① transparent layer, ② dark, ③ lesion itself and ④ surface. Since the previous methods of ultra-thin sectioning of enamel tend to produce artificial lesions, transmission electron microscopy has so far failed to provide information on these four layers of ultrastructural changes. This article describes an improved technique for thinning selected areas using argon ion light. This technique produces only minimal manual damage to normal enamel. This method can directly see the microscopic appearance of enamel, enamel crystals are lath-like, but can not determine the length. The authors selected 24 permanent teeth from early 15 to 30 years of age who had early similar caries (white lesions) and were routinely made into discs, which were then thinned with argon ion light for electron microscopic examination.