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目的:比较西红花酸(Crocetin)和西红花苷(Crocin)对损伤心肌保护作用的差异。方法:用血清药理学的方法,测定细胞培养液中LDH、CK、MDA,SOD的含量及细胞活力;同时,采用大鼠冠脉左前降支结扎心肌缺血模型,观察心电图变化,检测LDH、CK,心肌梗死面积及心肌组织病理变化。结果:西红花酸含药血清能显著降低血清和缺糖缺氧心肌细胞上清中LDH、CK、MDA的含量,提高SOD的含量,增强细胞的活力,ig西红花酸能降低冠脉结扎大鼠ECG的J点位移和心肌梗死面积百分率,减轻心肌组织病理改变;而西红花苷无此作用。结论:ig西红花酸均能明显减轻心肌损伤,治疗心肌缺血,西红花酸临床给药可以口服,而西红花苷治疗心肌缺血口服是一个不合适的给药途径。
Objective: To compare the protective effects of crocetin and crocin on myocardial injury. Methods: Serum pharmacology method was used to determine the contents of LDH, CK, MDA, SOD and cell viability in cell culture medium. Meanwhile, the model of myocardial ischemia was induced by left anterior descending coronary artery in rats. The changes of ECG, LDH, CK, myocardial infarction area and myocardial histopathological changes. Results: The crocetin-containing serum could significantly reduce the content of LDH, CK and MDA in the serum and hypoglycemic hypoxic cardiomyocyte supernatant, increase the content of SOD and increase the cell viability. The J point displacement and myocardial infarction area percentage of rat ECG were ligation, and the pathological changes of myocardial tissue were alleviated; crocidide did not. Conclusion: All crocetin can significantly reduce myocardial injury, the treatment of myocardial ischemia, crocetin clinical administration can be taken orally, while administration of crocintion of myocardial ischemia is an inappropriate route of administration.