论文部分内容阅读
Thymic nurse cells (TNCs) represent a unique microenvironment in the thymus for T cell maturation.In order toinvestigate the role of thymic nurse cells during T cell differentiation,a TNC clone,RWTE-1,which formed atypical complex with fetal thymocytes in vitro was established from normal Wistar rat.Hanging drop culturemethod was applied to reveal the interaction between TNCs and thymocytes.Our result revealed that eightypercent of immature CD4~-CD8~+ cells differentiated into CD4~+CD8~+ cells after a 12-hour hanging drop culture withRWTE-1.However,in a 12-hour culture of immature CD4~-CD8~+ cells with or without RWTE-1 supernatant,only30% of the cells differentiated into CD4~+CD8~+ cells spontaneously.This observation led to the conclusion thatRWTE-1 cell has the capacity to facilitate immature CD4~-CD8~+ thymocytes to differentiate into CD4~+CD8~+ T cellsby direct interaction.Cellular & Molecular Immunology.2005;2(4):301-305.
Thymic nurse cells (TNCs) represent a unique microenvironment in the thymus for T cell maturation. In order to investigate the role of thymic nurse cells during T cell differentiation, a TNC clone, RWTE-1, which formed atypical complex with fetal thymocytes in vitro was established from normal Wistar rat. Huangting drop culturemethod was applied to reveal the interaction between TNCs and thymocytes. Our results revealed that eightypercent of immature CD4 ~ -CD8 ~ + cells differentiated into CD4 ~ + CD8 ~ + cells after a 12-hour hanging drop culture with RT-1 supernatant, only30% of the cells differentiated into CD4 ~ + CD8 ~ + cells spontaneously.This observation led to the conclusion that RWTE-1 cells have the capacity to facilitate immature CD4 ~ -CD8 ~ + thymocytes to differentiate into CD4 ~ + CD8 ~ + T cells by direct interaction. Cellular & Molecular Immunology. 2005; 2 (4): 301-305.