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利用HPLC-MSn检测罂粟碱及其在大鼠体内(大鼠粪便)和体外(肝微粒体,肠道菌)代谢物。体内与体外的代谢物经C18小柱进行富集和纯化后,直接采用优化的HPLC-ESI/ITMSn方法对样品进样分析。以罂粟碱标准品为对象优化高效液相色谱/电喷雾离子阱质谱(HPLC-ESI/ITMSn)实验条件,分析总结其电喷雾质谱的一级电离规律和多级质谱裂解规律,作为罂粟碱大鼠体内与体外代谢物结构分析的依据。代谢物的结构推导主要依据代谢物的色谱保留时间及其电喷雾离子阱质谱HPLC-ESI/ITMSn电离规律。在大鼠粪便中有原药及其8种代谢产物。体外代谢物检测到原药的脱甲基和羟基化。
Papaverine and its metabolites in rats (rat faeces) and in vitro (liver microsomes, gut bacteria) were detected by HPLC-MSn. After in vivo and in vitro metabolites were enriched and purified by a C18 cartridge, the samples were directly analyzed by HPLC-ESI / ITMSn method. To optimize the HPLC-ESI / ITMSn experimental conditions for papaverine standard, the first-order ionization and multi-level mass spectrometry of the electrospray ionization mass spectrometry (ESI-MS / MS) The Basis of Structural Analysis of Metabolites in Rats and in. The structure of the metabolites was deduced mainly based on the chromatographic retention time of the metabolites and their ionization ion trap mass spectrometry HPLC-ESI / ITMSn ionization law. In the rat feces with the original drug and its eight metabolites. In vitro metabolites demethylation and hydroxylation of the prodrugs were detected.