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目的检测果蔬饮料胡萝卜汁抗突变作用的效应情况,为开发果蔬饮品提供实验数据。方法利用鼠伤寒沙门菌营养缺陷型Ames点实验改良法,37℃恒温培养48h。计算在纸圈内所产生的回复变菌落数,据测试物和溶剂对照的细菌回复变集落数的差异,判断是否有抑制突变的作用。结果TA98,TA100菌株均在正常范围内。未观察到所有实验的样品有致突变作用现象。结论新黑田胡萝卜汁和双歧因子型2%醋酸胡萝卜汁对MNNG诱导TA100,新黑田胡萝卜汁+2%醋和农家胡萝卜汁对AFB1诱导TA98+S9的抑制率较高,剂量效应关系明显,新黑田胡萝卜汁和新黑田胡萝卜汁+2%醋对AFB1、新黑田胡萝卜汁对BAP诱导A100+S9也有较高的抑制,但不构成剂量效应关系。
Objective To detect the effect of carrot juice on the mutagenicity of fruits and vegetables and provide experimental data for the development of fruit and vegetable drinks. Methods Salmonella typhimurium malnutrition type Ames point experiment improved method, 37 ℃ constant temperature 48h. The number of replicate colonies produced in the paper circle was calculated. According to the difference of the number of colonies returned by bacteria in the test substance and the solvent control, it was determined whether there was any effect of suppressing the mutation. Results TA98, TA100 strains were within the normal range. No experimental samples were observed to be mutagenic. CONCLUSION New Radix et Rhizoma carrot juice and bifidogenic 2% acetic acid carrot juice have a higher dose-effect relationship with TANi-TA9 and TA9 + S9 induced by MNNG, TAKARITA carrot juice + 2% vinegar and farm carrot juice , New Kuroda carrot juice and new Kuroda carrot juice + 2% vinegar on AFB1, New Kuroda carrot juice on BAP-induced A100 + S9 also higher inhibition, but does not constitute a dose-response relationship.