Ohjective:To assess molecular characterization,distribution,seasonal activities of sandfly species and Leishmania parasites infecting them for this zoonotic cutaneous leishmaniasis focus.Methods:The collections were carried out in 2009-2011 using CDC traps,Sticky Papers and manual aspirator in and around the villages in Abarkouh district.Individual sandflies were characterized by PCR amplification and sequencing of fragments of their mitochondrial cytochrome b gene.Leishmania parasite infections within sandflies were performed by targeting Cyt b,ITS-rDNA,k-DNA and microsatellite genes.Results:The PCR assays detected only Leishmania major(L.major).All infections(30)were found in the abundant and widespread vector Phlebolomus papalasi(P.papatasi).Small numbers of other sandfly species were also screened for infections,but none was found.Sergentomyia sinloni and P.papalasi were the predominant members in all locations of this district and in all habitats throughout the trapping season.Only five other sandfly species were found,namely Phlebolomus ansari,Phlebotomus caucasicus,Phlebotomus sergenti,Sergentomyia dentata and Sergentomyia merviney.Conclusions:In the current survey,the only infections detected are of L.major in females of P.papatasi(30 out of190).The rates of infection of P.papalasi by L.major are not significantly different in compare with other locations in Iran with no diversity of parasite strains.Zoonotic cutaneous leishmaniasis may have emerged only recently in Abarkouh district,and the reason may well be the instability of the transmission cycles there. Ohjective: To assess molecular characterization, distribution, seasonal activities of sandfly species and Leishmania parasites infecting them for this zoonotic cutaneous leishmaniasis focus. Methods: The collections were carried out in 2009-2011 using CDC traps, Sticky Papers and manual aspirator in and around the villages in Abarkouh district. Individual sandflies were characterized by PCR amplification and sequencing of fragments of their mitochondrial cytochrome b gene. Leishmania parasite infections within sandflies were performed by targeting Cyt b, ITS-rDNA, k-DNA and microsatellite genes. Results: The PCR Assays detected only Leishmania major (L. major) .All infections (30) were found in the abundant and widespread vector Phlebolomus papalasi (P.papatasi) .Small numbers of other sandfly species were also screened for infections, but none was found .Sergentomyia sinloni and P.papalasi were the predominant members in all locations of this district and in all habitats throughout the trapping season.Onl y five other sandfly species were found, namely Phlebolomus ansari, Phlebotomus caucasicus, Phlebotomus sergenti, Sergentomyia dentata and Sergentomyia merviney. Conlusions: In the current survey, the only infections detected are of L. major in females of P. papatasi (30 out of 190 ) The rates of infection of P.papalasi by L.major are not significantly different in compare with other locations in Iran with no diversity of parasite strains. Zoonotic cutaneous leishmaniasis may have only recently in Abarkouh district, and the reason may well be the instability of the transmission cycles there.